fig4

Figure 4. Rapamycin impacts the inhibitory effect of MAGEA3 on VEGF expression. (A and B) Western blot and quantification of phosphorylated mTOR protein level and total mTOR protein level after knocking down MAGEA3 in HCT116 cells and overexpression of MAGEA3 in HT29 cells; (C) mRNA levels of VEGF in shNC and shMAGEA3 HCT116 cell lines treated or untreated with rapamycin (rapa); (D) mRNA levels of VEGF in EGFP and MAGEA3 overexpression HT29 cell lines treated or untreated with rapamycin. ns: no significance, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. shNC: shRNA for negative control; MAGEA3-OE: MAGEA3 overexpression; rapa: rapamycin.