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Figure 3. MAGEA3 inhibits VEGF expression in CRC. (A) The mRNA level of VEGF in the shNC and shMAGEA3 groups of HCT116 cells, and the EGFP and MAGEA3-OE groups of HT29 cells; (B and C) Representative images and quantification of Western blot of VEGF in medium of the shNC and shMAGEA3 groups of HCT116 cells, and the EGFP and MAGEA3-OE groups of HT29 cells cultured in serum-free medium for 24 h. Ponceau S staining was used as an internal loading control alongside Western blot detection of VEGF; (D) Representative immunohistochemistry (IHC) staining images of MAGEA3 and VEGF in human colorectal cancer (scale bar: 100 μm); (E) Correlation between the IHC scores of MAGEA3 and VEGF in human CRC. ^*P < 0.05, ^**P < 0.01, ^****P < 0.0001. shNC: shRNA for negative control; MAGEA3-OE: MAGEA3 overexpression; IHC: immunohistochemistry.