fig4

Figure 4. SyBV treatment is more effective in small early-stage melanoma. Tumor growth curve of mice with small (10-30 mm³) vs. larger (50-100 mm³) initial melanoma sizes intratumorally immunized with SyBV (10¹⁰). n = 5/group. An anti-PD-1 antibody (100 µg) was given intraperitoneally 1 day prior to immunization with SyBV. Data are presented as the mean ± SEM. ^***P < 0.001 by two-way ANOVA with Tukey’s post-test vs. the sham group at the indicated time points (Day 7 shown). SyBV: Synthetic bacterial vesicle; PD-1: programmed cell death protein 1; SEM: standard error of the mean; ANOVA: analysis of variance.