fig1

Figure 1. An overview of the experimental design adopted for each of the three experiments conducted. (A) Experiment 1 evaluated whether sEVs carried OXT and, if so, whether the OXT was carried inside or outside the sEVs; (B) Experiment 2 investigated whether the amount of OXT in sEVs differs between ejaculate fractions; (C) Experiment 3 investigated whether the concentration of OXT in seminal EVs was related to in vivo fertility outcomes in boars used in artificial insemination programs. Created in BioRender (Roca, J., 2025; https://BioRender.com/hqwnptw). Although some symbols were obtained from BioRender, Figure 1 was composed using Microsoft PowerPoint. SP: Seminal plasma; sEVs: seminal extracellular vesicles; SRF-P1: the first 10 mL of the sperm-rich fraction of the ejaculate (SRF); SRF-P2: the remainder of the SRF; Post-SRF: the ejaculate fraction that is poor in spermatozoa; FR: farrowing rate; LS: litter size.