fig6

Figure 6. Anticancer effect of agarose/Fe3O4 hydrogels. Live/dead staining of MDA-MB-231-Luc cells cultured with the agarose/Fe3O4 hydrogel discs via sitting (A), transwell (C), and adhesion (E) modes before and after AMF irradiation (live cell: green fluorescence, dead cells: red fluorescence). Quantified cell viability during culture without or with agarose/Fe3O4 hydrogel discs via sitting (B), transwell (D), and adhesion (F) modes before and after AMF irradiation. Cell viability was normalized to that cultured with agarose hydrogel without free Fe3O4 NPs before AMF irradiation. Data are the mean ± SD (n = 3). Significant difference: *P < 0.05,