fig5

Figure 5. Anticancer effect of free Fe₃O₄ NPs. Live/dead staining of MDA-MB-231-Luc cells cultured without or with free Fe₃O₄ NPs before and after AMF irradiation (live cell: green fluorescence, dead cells: red fluorescence) (A). Quantified cell viability during culture without or with free Fe₃O₄ NPs before and after AMF irradiation (B). Cell viability was normalized to that cultured with PBS without free Fe₃O₄ NPs before AMF irradiation. Data are the mean ± SD (n = 3). Significant difference: ***P < 0.001. N.S. : no significant difference.