fig3

Figure 3. FASN expression is upregulated in TNNI3^R186Q+/+ mouse hearts. (A) FASN protein levels measured by Western blot were detected in Tnni3^+/+ and Tnni3^R186Q/R186Q mice ventricular myocardium and a representative experiment is shown (n = 3 per group). (B) The Western blot of FASN protein levels is expressed as the means ± SEM. (C) The expression levels of FASN mRNA were detected by qRT-PCR in the ventricular myocardium of Tnni3^+/+ and Tnni3^R186Q/R186Q mice (n = 3 per group). (D) Protein expression levels of fatty acid synthesis related proteins were detected by Western blot in Tnni3^+/+ and Tnni3^R186Q/R186Q mice ventricular myocardium (n = 3 per group). (E) Protein expression levels of fatty acid β-oxidation related proteins were detected by Western blot in Tnni3^+/+ and Tnni3^R186Q/R186Q mouse ventricular myocardium (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001 and ns: not significant. The statistical test was done with unpaired T-test.