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Figure 2. Immunophenotype of BMSCs in analyzed media. (A and B) BMSCs in the different media were positive for CD44, CD73, CD90, and CD146, while negative for CD271 and CD45. In the histograms, only BMSCs in the F condition are shown for clarity. In the Abs staining plots, BMSCs were gated using the FSC vs. SSC. Data shown are from a representative donor; (C) Violin plot of CD146 positivity, N = 8 donors. Median and quartiles are shown as dashed and dotted lines, respectively. BMSCs: Bone marrow-derived mesenchymal stromal cells; F: fetal bovine serum; P: platelet lysate; S/X: serum/xeno free; FSC: forward scatter; SSC: side scatter. ****P-value ≤ 0.0001.