fig6

Figure 6. NPC exposure to EV-Aβ₄₀ causes dysregulation of mitochondrial fusion and fission machinery. (A) Total mRNA expression was assessed for fusion modulators: OPA1, Mfn1, and Mfn2. The total protein expression levels were assessed along with representative western blots for fusion proteins (B) OPA1, (C) Mfn1, and (D) Mfn2. GAPDH was used as a housekeeping control for all experiments. Additionally, modulators of fission, Fis1, Drp1, and Mff, were assessed via qPCR (E). Total protein expression and representative western blots for mitochondrial fission proteins (F) Mff, (G) Drp1, and (H) Fis1 were quantified. Bcl-2 was probed for (I) total mRNA gene expression and (J) total protein expression by western blotting. Bar graphs indicate mean ± SEM. Statistical analysis was performed using one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, n = 3-6 independent experiments per group. EV: Extracellular vesicles; NPC: neural progenitor cell; SEM: standard error of the mean.