fig3

Figure 3. Electron microscopy pictures from FBS-free medium (A) and EVs isolated with UC ×2 (B), SEC-UC (C), CP (D), CP-SEC-UC (E), UF-SEC-UC (F), UC-SEC-CP (G), UC-SEC-UF (H), and UC-SEC-UC (I). Black arrow: cup-shaped particles (vesicles); white circles: non-vesicles particles; black asterisks: undefined aggregates. FBS: Fetal bovine serum; EVs: extracellular vesicles; UC: ultracentrifugation; SEC: size exclusion chromatography; CP: chemical precipitation.