fig3

Figure 3. Effects of chronic β-FNA on viability of NHA. Cells were cultured in growth medium containing 3 µmol/L β-FNA for 24 h; the medium was then replaced with serum-free medium containing β-FNA for an additional 48 h. IL-1β (3 ng/mL) was added to cultures for the final 24 h. Cell viability was assessed using the MTT assay. Data are presented as mean ± SEM (n = 8). ANOVA did not reveal any significant differences. MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; SEM: standard error of the mean; β-FNA: beta-funaltrexamine; NHA: normal human astrocytes; IL-1β: interleukin-1β