fig2

Figure 2. Pyrosequencing was used for quantitative analysis of O6-methylguanine DNA methyltransferase promoter methylation. Pyrogram demonstrating (a) an unmethylated and (b) methylated glioblastoma tissue. Each colored box includes one of the four studied CpG positions (CpGs 9-12). The incorporation of the bases guanine and adenine represent the methylated and unmethlylated fractions, respectively. The percentages given in both pyrograms reflect the methylated fractions (fractions over 10% define a methylated sample)